A total of 196 samples, made up of 130 clinical (nosocomial) samples and 66 non-clinical (community) samples were examined for Staphylococcus aureus. Antibiotic resistance profiles of the S. aureus isolates were determined. Also homogeneity/heterogeneity of the clinical and community strains was determined by comparing their plasmid profiles. Out of the 196 samples screened, 53 (27%) yielded Staphylococcus aureus. Of the 130 clinical samples, 41 (32%) were positive for S. aureus. Of these, 21 (51%) were from formites, while 20 (49%) were from patients. There was a significantly(p<0.05) lower incidence of S. aureus in the non-clinical samples with only 12 out of 66 samples (18%) yeilding the organism. Also the clinical S. aureus isolates were significantly(p<0.05) more resistant to antibiotics, particularly the older antibiotics, than the community isolates. Plasmid profiling showed, however, that there was a great degree of homogeneiyy between the clinical and community S. aureus isolates suggesting that it is mostly the same strain of S. aureus that is circulating in both the nosocomial and community environments in Nsukka metropolis.