Distinguishing the sites of pre-rRNA synthesis and accumulation in Ehrlich tumor cell nucleoli.

@article{Thiry1991DistinguishingTS,
  title={Distinguishing the sites of pre-rRNA synthesis and accumulation in Ehrlich tumor cell nucleoli.},
  author={Marc Thiry and Guy Goessens},
  journal={Journal of cell science},
  year={1991},
  volume={99 ( Pt 4)},
  pages={759-67}
}
The precise location of transcribing rRNA genes within Ehrlich tumor cell nucleoli has been investigated using two approaches: high-resolution autoradiography of cells pulse-labelled with tritiated uridine, varying the exposure time, and in situ-in vitro transcription coupled with an immunogold labelling procedure. When autoradiographic preparations are exposed for a short time, silver grains are found associated almost exclusively with interphasic cell nucleoli. Labelling of extranucleolar… CONTINUE READING

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