Distinguishing between protein dynamics and dye photophysics in single-molecule FRET experiments.

Abstract

Förster resonance energy transfer (FRET) efficiency distributions in single-molecule experiments contain both structural and dynamical information. Extraction of this information from these distributions requires a careful analysis of contributions from dye photophysics. To investigate how mechanisms other than FRET affect the distributions obtained by… (More)
DOI: 10.1016/j.bpj.2009.12.4322

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