Distinguishing between folate receptor-alpha-mediated transport and reduced folate carrier-mediated transport in L1210 leukemia cells.

@article{Spinella1995DistinguishingBF,
  title={Distinguishing between folate receptor-alpha-mediated transport and reduced folate carrier-mediated transport in L1210 leukemia cells.},
  author={Michael J. Spinella and Kevin E. Brigle and Europa Sierra and I. David Goldman},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 14},
  pages={7842-9}
}
L1210 leukemia cells transport reduced folates and methotrexate via a well defined reduced folate carrier system and, in the absence of low folate selective pressure, do not express an alternate endocytotic route mediated by cell surface folate receptors. This laboratory previously described an L1210 leukemia cell line, MTXrA, with acquired resistance to methotrexate (MTX) due to the loss of mobility of the reduced folate carrier. We now report on the transfection of MTXrA with a cDNA encoding… CONTINUE READING