Distinctive properties of mammalian DNA polymerases.

  title={Distinctive properties of mammalian DNA polymerases.},
  author={Dipak K Dube and T. A. Kunkel and G M Seal and L. A. Loeb},
  journal={Biochimica et biophysica acta},
  volume={561 2},
DNA polymerase-alpha and -beta can be distinguished from one another by the differential effects of N-ethylmaleimide, KCl, ara-CTP and temperature, as well as on the basis of sedimentation. The sensitivity of DNA polymerase-beta to elevated temperatures as compared to DNA polymerase-alpha provides a new means of distinguishing between these two enzymes even in crude extracts and a possible probe for determining their function. DNA polymerase-alpha and -beta share several properties in common… CONTINUE READING