Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.

@article{Lokareddy2015DistinctivePO,
  title={Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.},
  author={Ravi Kumar Lokareddy and Rizqiya A Hapsari and Mathilde van Rheenen and Ruth A Pumroy and Anshul Bhardwaj and Anton Steen and Liesbeth M Veenhoff and Gino Cingolani},
  journal={Structure},
  year={2015},
  volume={23 7},
  pages={1305-1316}
}
Targeting of ER-synthesized membrane proteins to the inner nuclear membrane (INM) has long been explained by the diffusion-retention model. However, several INM proteins contain non-classical nuclear localization signal (NLS) sequences, which, in a few instances, have been shown to promote importin α/β- and Ran-dependent translocation to the INM. Here, using structural and biochemical methods, we show that yeast INM proteins Heh2 and Src1/Heh1 contain bipartite import sequences that associate… CONTINUE READING