Distinct substrate specificities of Arabidopsis DCL3 and DCL4

@inproceedings{Nagano2014DistinctSS,
  title={Distinct substrate specificities of Arabidopsis DCL3 and DCL4},
  author={Hideaki Nagano and Akihito Fukudome and Akihiro Hiraguri and Hiromitsu Moriyama and Toshiyuki Fukuhara},
  booktitle={Nucleic acids research},
  year={2014}
}
In Arabidopsis thaliana, Dicer-like 3 (DCL3) and Dicer-like 4 (DCL4) cleave long, perfect double-stranded RNAs (dsRNAs) into 24 and 21 nucleotides (nt) small interfering RNAs, respectively, which in turn function in RNA-directed DNA methylation and RNA interference, respectively. To reveal how DCL3 and DCL4 individually recognize long perfect dsRNAs as substrates, we biochemically characterized DCL3 and DCL4 and compared their enzymatic properties. DCL3 preferentially cleaves short dsRNAs with… CONTINUE READING

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