Distinct regions of Sp1 modulate DNA binding and transcriptional activation.

@article{Kadonaga1988DistinctRO,
  title={Distinct regions of Sp1 modulate DNA binding and transcriptional activation.},
  author={James T. Kadonaga and Albert J. Courey and J Ladika and Robert Tjian},
  journal={Science},
  year={1988},
  volume={242 4885},
  pages={1566-70}
}
Sp1 is a sequence-specific DNA binding protein that activates RNA polymerase II transcription from promoters that contain properly positioned GC boxes. A series of deletion mutants of Sp1 were expressed in Escherichia coli and used to identify separate regions of the protein that are important for three different biochemical activities. The sequence-specificity of DNA binding was conferred by Zn(II) fingers, whereas a different region of Sp1 appeared to regulate the affinity of DNA binding. The… CONTINUE READING