Distinct rates of palmitate turnover on membrane-bound cellular and oncogenic H-ras.

@article{Baker2003DistinctRO,
  title={Distinct rates of palmitate turnover on membrane-bound cellular and oncogenic H-ras.},
  author={Tara L Baker and Hui Zheng and Joy A Walker and Jonathan L. Coloff and Janice E. Buss},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 21},
  pages={19292-300}
}
H-Ras displays dynamic cycles of GTP binding and palmitate turnover. GTP binding is clearly coupled to activation, but whether the palmitoylated COOH terminus participates in signaling, especially when constrained by membrane tethering, is unknown. As a way to compare COOH termini of membrane-bound, lipid-modified H-Ras, palmitate removal rates were measured for various forms of H-Ras in NIH 3T3 cells. Depalmitoylation occurred slowly (t(1/2) approximately 2.4 h) in cellular (H-RasWT) or… CONTINUE READING