Distinct phosphatases mediate the deactivation of the DNA damage checkpoint kinase Rad53.

@article{Travesa2008DistinctPM,
  title={Distinct phosphatases mediate the deactivation of the DNA damage checkpoint kinase Rad53.},
  author={Anna Travesa and Alba Duch and David G. Quintana},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 25},
  pages={17123-30}
}
The DNA damage checkpoint regulates DNA replication and arrests cell cycle progression in response to genotoxic stress. In Saccharomyces cerevisiae, the protein kinase Rad53 plays a central role in preventing genomic instability and maintaining viability in the presence of replication stress and DNA damage. Activation of Rad53 depends on phosphorylation by the upstream kinase Mec1, followed by autophosphorylation on multiple residues. Also critical for cell viability, the molecular mechanism of… CONTINUE READING