Distinct phenotype of E-selectin-deficient mice. E-selectin is required for slow leukocyte rolling in vivo.

@article{Kunkel1996DistinctPO,
  title={Distinct phenotype of E-selectin-deficient mice. E-selectin is required for slow leukocyte rolling in vivo.},
  author={Eric J. Kunkel and Klaus Ley},
  journal={Circulation research},
  year={1996},
  volume={79 6},
  pages={
          1196-204
        }
}
Leukocyte capture and rolling are mediated by calcium-dependent lectins expressed on most leukocytes (L-selectin) and the vascular endothelium (P- and E-selectin). To study the role of the selectins during inflammation, we have investigated leukocyte rolling in venules of tumor necrosis factor-alpha (TNF-alpha)-treated mouse cremaster muscles in wild-type mice and gene-targeted mice with homozygous deficiency for L-, P-, or E-selectin (L-/-, P-/-, or E-/-, respectively). TNF-alpha treatment… Expand
Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion.
TLDR
It is concluded that L-selectin can mediate rolling that results in sufficient leukocyte recruitment to account for the robust inflammatory response seen in E-/P- mice at later times. Expand
Importance of E-selectin for firm leukocyte adhesion in vivo.
TLDR
It is concluded that slow leukocytes rolling through E-selectin results in long transit times, which are essential for efficient leukocyte adhesion in response to a local chemotactic stimulus. Expand
Selectin-independent leukocyte rolling and adhesion in mice deficient in L-, E-, and P-selectin and ICAM-1
  • S. Forlow, K. Ley
  • Chemistry, Medicine
  • Proceedings of the First Joint BMES/EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Meeting of the Biomedical Engineering Society (Cat. N
  • 1999
TLDR
Some leukocyte adherence can occur without rolling through selectin and /spl alpha//sub 4/ integrin-independent mechanisms, and this recruitment mechanism is confined to venular branch points. Expand
A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions.
TLDR
E-selectin mediates PS GL-1-dependent and independent rolling and the latter can be down-regulated by systemic activation and can replace PSGL-1 to support the development of inflammation. Expand
Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice.
TLDR
The occurrence of skin and pulmonary disease in E/P double-Mutant mice but not E/L/P triple-mutant mice suggests that deficiency of L-selectin alters the inflammatory response in E-P mutants. Expand
P-selectin Glycoprotein Ligand-1 Mediates L-Selectin–dependent Leukocyte Rolling in Venules
TLDR
It is concluded that leukocyte-expressed PSGL-1 serves as the main L-selectin ligand in inflamed postcapillary venules, providing a molecular mechanism for the inflammatory defects seen in L- selectin–deficient mice. Expand
Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment.
TLDR
The generation and comparative phenotype of mice lacking one, two, or three selectins after sequential ablation of the murine genes encoding P-, E-, and L-selectins are described and a preeminent role for P-selectin in regulating leukocyte behavior in mice is indicated. Expand
Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin.
TLDR
It is concluded that any one of the selectins can support some neutrophils recruitment but eliminating all three selectins significantly impairs neutrophil recruitment. Expand
P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo.
TLDR
The results suggest that P-selectin-PSGL-1 interaction alone is sufficient to mediate rolling in vivo and that E- selectin- PSGL- 1 interaction supports slow rolling. Expand
P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo
Selectin-dependent rolling is the earliest observable event in the recruitment of leukocytes to inflamed tissues. Several glycoproteins decorated with sialic acid, fucose, and/or sulfate have beenExpand
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It is demonstrated that the majority of neutrophil migration in both models requires an endothelial selectin but that E-selectin and P- selectin are functionally redundant, which has important implications in the use of selectin antagonists in the treatment of inflammatory disease. Expand
L-selectin-deficient mice have impaired leukocyte recruitment into inflammatory sites
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It is demonstrated that L-selectin plays a prominent role in leukocyte homing to nonlymphoid tissues during inflammation and that blocking this process can be beneficial during pathological inflammatory responses. Expand
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Whereas P-selectin is known to be involved in early stages of an inflammatory response, the results indicate that it is additionally responsible for leukocyte rolling and macrophage recruitment in more prolonged tissue injury. Expand
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Data show that P- selectin is important during the initial induction of leukocyte rolling after tissue trauma, and at later time points and in TNF-alpha-treated preparations, rolling is largely L-selectin dependent. Expand
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TLDR
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TLDR
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TLDR
ESL-1, with a structure essentially identical to that of a receptor, thus functions as a cell adhesion ligand of E-selectin, and fucosylation of ESL-1 is imperative for affinity isolation with E- selectin-IgG. Expand
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