Distinct isoprenoid origins of cis- and trans-zeatin biosyntheses in Arabidopsis.

@article{Kasahara2004DistinctIO,
  title={Distinct isoprenoid origins of cis- and trans-zeatin biosyntheses in Arabidopsis.},
  author={Hiroyuki Kasahara and Kentaro Takei and Nanae Ueda and Shojiro Hishiyama and Tomoyuki Yamaya and Yuji Kamiya and Shinjiro Yamaguchi and Hitoshi Sakakibara},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 14},
  pages={
          14049-54
        }
}
Plants produce the common isoprenoid precursors isopentenyl diphosphate and dimethylallyl diphosphate (DMAPP) through the methylerythritol phosphate (MEP) pathway in plastids and the mevalonate (MVA) pathway in the cytosol. To assess which pathways contribute DMAPP for cytokinin biosynthesis, metabolites from each isoprenoid pathway were selectively labeled with (13)C in Arabidopsis seedlings. Efficient (13)C labeling was achieved by blocking the endogenous pathway genetically or chemically… CONTINUE READING

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