Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems.

@article{Yuan2010DistinctGC,
  title={Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems.},
  author={Jing Yuan and Patrick O'Donoghue and Alex Ambrogelly and Sarath B. Gundllapalli and Rachael L Sherrer and Sotiria Palioura and Miljan Simonovi{\'c} and Dieter S{\"o}ll},
  journal={FEBS letters},
  year={2010},
  volume={584 2},
  pages={342-9}
}
Selenocysteine and pyrrolysine, known as the 21st and 22nd amino acids, are directly inserted into growing polypeptides during translation. Selenocysteine is synthesized via a tRNA-dependent pathway and decodes UGA (opal) codons. The incorporation of selenocysteine requires the concerted action of specific RNA and protein elements. In contrast, pyrrolysine is ligated directly to tRNA(Pyl) and inserted into proteins in response to UAG (amber) codons without the need for complex re-coding… CONTINUE READING