Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans

@article{Pak2007DistinctPO,
  title={Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans},
  author={Julia Pak and A. Fire},
  journal={Science},
  year={2007},
  volume={315},
  pages={241 - 244}
}
RNA interference (RNAi) is a phylogenetically widespread gene-silencing process triggered by double-stranded RNA. In plants and Caenorhabditis elegans, two distinct populations of small RNAs have been proposed to participate in RNAi: “Primary siRNAs” (derived from DICER nuclease-mediated cleavage of the original trigger) and “secondary siRNAs” [additional small RNAs whose synthesis requires an RNA-directed RNA polymerase (RdRP)]. Analyzing small RNAs associated with ongoing RNAi in C. elegans… Expand
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