Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane.

@article{Nilsson2000DistantDS,
  title={Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane.},
  author={Ingmarie Nilsson and Sebastian Witt and H{\'e}l{\`e}ne Kiefer and Ismael Mingarro and Gunnar von Heijne},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 9},
  pages={6207-13}
}
We have studied the membrane insertion of ProW, an Escherichia coli inner membrane protein with seven transmembrane segments and a large periplasmic N-terminal tail, into endoplasmic reticulum (ER)-derived dog pancreas microsomes. Strikingly, significant levels of N-tail translocation is seen only when a minimum of four of the transmembrane segments are present; for constructs with fewer transmembrane segments, the N-tail remains mostly nontranslocated and the majority of the molecules adopt an… CONTINUE READING

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6207–6213 Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane

  • V. Goder, C. Bieri, M. Spiess
  • J. Cell Biol. 147,
  • 1999

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