Dissolved free amino acids in coastal seawater using a modified fluorometric method

  title={Dissolved free amino acids in coastal seawater using a modified fluorometric method},
  author={K. Tada and Mitsutoshi Tada and Y. Maita},
  journal={Journal of Oceanography},
The method of Parsonset al. (1984) for measuring dissolved free amino acids (DFAA) in coastal seawater was modified. We found considerable interference in DFAA determination from ammonia dissolved in coastal seawater, although the interference of urea could be ignored. For DFAA analysis for coastal seawater samples, ammonia determinations for the same sample are needed to correct DFAA values. For coastal surface seawater samples from all over the Seto Inland Sea, Japan, values of DFAA ranged… Expand

Figures and Tables from this paper

Determination of picomolar dissolved free amino acids along a South Atlantic transect using reversed-phase high-performance liquid chromatography
Modifications to methodology using o-phthaldialdehyde derivatization and reversed phase high performance liquid chromatography (HPLC) that provide a 30-fold improvement in sensitivity enabling the detection of 15 amino acids in seawater with a limit of detection as low as 10 pmol L − 1 with accuracy and precision of better than 10%. Expand
Temporal dynamics and depth variations of dissolved free amino acids and polyamines in coastal seawater determined by high-performance liquid chromatography
Short-chained aliphatic polyamines (PAs) are a class of labile dissolved organic nitrogen (DON) that has biogeochemical similarities to dissolved free amino acids (DFAAs). Here we investigated theExpand
The determination of total dissolved free primary amines in seawater: Critical factors, optimized procedure and artefact correction
The paper reviews the methods described in the literature for the determination of total dissolved free primary amines (TDFPA) by fluorescence. A wide set of reaction conditions can be found, butExpand
Uptake of dissolved free amino acids by four cold-water coral species from the Mediterranean Sea
It is demonstrated that dissolved organic matter, in the form of dissolved free amino acids (DFAA), is actively absorbed by four cold-water coral species from the Mediterranean Sea, and can supply a significant amount of carbon and nitrogen to the corals, especially during periods when particulate food is scarce. Expand
Free polyamine concentrations in coastal seawater during phytoplankton bloom
Investigation of the concentrations of polyamines together with inorganic nutrients during a summer bloom period in the Seto Inland Sea of Japan found putrescine and spermidine seemed to be significant compounds in dissolved organic nitrogen in coastal seawater. Expand
Detection of free polyamine in coastal seawater using ion exchange chromatography
Polyamines are widely distributed in living organisms and are known to be essential elements for normal growth and development. Although they may affect phytoplankton growth their existence andExpand
Nitrogen dynamics in the Irish Sea and adjacent shelf waters: An exploration of dissolved organic nitrogen
Abstract Relatively little is known about dissolved organic nitrogen (DON) in the marine environment because research has historically focused on dissolved inorganic nitrogen (DIN). In this study weExpand
Short-term uptake of dissolved organic nitrogen by an axenic strain of Ulva pertusa (Chlorophyceae) using [15]N isotope measurements
Results show that U. pertusa can take up urea and alanine directly without bacterial decomposition to inorganic nitrogen, and has the potential to regulate DON fluxes and transformations in coastal environments. Expand
Sediment amino acids as indicators of anthropogenic activities and potential environmental risk in Erhai Lake, Southwest China.
The results showed that the dramatic increase in THAAs in the sediment cores indicated that there was a huge potential source of labile nitrogen for the overlying water under certain environmental conditions, and correlation analysis suggested that the release of THAas was negatively correlated with pH, whereas positively correlated with bacterial number and degree of OM mineralization. Expand
Growth responses of Ulva prolifera to inorganic and organic nutrients: Implications for macroalgal blooms in the southern Yellow Sea, China
The marine macrophyte Ulva prolifera is the dominant green-tide-forming seaweed in the southern Yellow Sea, China. Here we assessed, in the laboratory, the growth rate and nutrient uptake responsesExpand


The determination of α-amino acids in seawater using a fluorimetric analyser
Abstract A method is described for the detection of free α-amino acids in the picomole range in seawater samples of less than 100 ml volume. The modifications made to a standard amino acid analyserExpand
Nitrogen sources for the growth of marine microalgae: role of dissolved free amino acids
Results from laboratory and field studies suggest that maximum rates of DFAA uptake would occur in dark conditions in waters depleted of dissolved inorganic nitrogen, and Turbid estuaries and coastal waters would also be environments likely to induce a significant uptake of amino acids. Expand
Vertical distribution of total dissolved nitrogen and dissolved organic nitrogen in seawater.
In order to reveal the vertical distribution of dissolved organic nitrogen (DON) in seawater, a critical examination is made of two the analytical methods for the constituents, i.e. the persulfateExpand
Free amino acids in marine rains: evidence for oxidation and potential role in nitrogen cycling
Previous studies of dissolved organic nitrogen (DON) in precipitation have addressed various aspects of nutrient transport and global nitrogen cycling1. In most of these studies however, the detailedExpand
Determination of urea in natural water by an improved Newell method
The method for determination of urea in sea water proposed by Newell et al. was re-examined in detail and partly modified. The following method is recommended.To 25 mlof a sample solution are added 3Expand
Comparison of the amino acid concentrations at the incurrent and excurrent siphons of the clam, Spisula, indicated that removal of glycine took place in the mantle cavity of this animal, suggesting capacity for uptake was not limited to filter-feeding animals. Expand
Diel Production and Microheterotrophic Utilization of Dissolved Free Amino Acids in Waters Off Southern California
Diel patterns of dissolved free amino acid concentration and microheterotrophic utilization were examined in the spring and fall of 1981 in euphotic waters from the base of the mixed layer off the southern California coast to study the effects of primary production and chlorophyll a concentrations. Expand
Uptake of some dissolved organic nutrients by the Sydney rock oyster Saccostrea commercialis
The efficiency of absorption as measured by the nutrient accumulation factor in oyster tissues was dependent on the nutrient concentrations in estuarine water. Expand
Standing stocks and production rates of phytoplankton and abundance of bacteria in the Seto Inland Sea, Japan
The disparity between Chla and bacterial density in Suo Nada suggests that bacterial abundance can be controlled by the availability of substrates other than phytoplankton exudate. Expand
Amino acid fluxes to and from seawater in axenic veliger larvae of the bivalve (Crassostrea gigas)
The overall contribution of dissolved amino acids will depend on the substrate concentration encountered by the larvae in nature, which could account for 100 % of their oxidative needs by the uptake of amino acids from seawater. Expand