• Medicine
  • Published in Acta dermato-venereologica 1976

Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.

@article{Hentzer1976DissociationOH,
  title={Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.},
  author={B. Hentzer and Takasi Kobayasi},
  journal={Acta dermato-venereologica},
  year={1976},
  volume={56 1},
  pages={
          19-25
        }
}
Primary cultures of epidermal cells from human adult skin were dissociated by dithiothreitol (DTT) with subsequent trypsinization. After treatment with disulfide reducing agents, the desmosomes were either destroyed or showed a homogeneous ultrastructure. Trypsin separated the homogeneous desmosomes, whereas cell membranes, organelles, and nuclei remained undamaged. Trypsin did not influence normal desmosomes. A suspension of human epidermal cells was recultivated in new flasks. Two types of… CONTINUE READING