Dissociation of chicken blastoderm for examination of apoptosis and necrosis by flow cytometry.

@article{Hamidu2010DissociationOC,
  title={Dissociation of chicken blastoderm for examination of apoptosis and necrosis by flow cytometry.},
  author={J. A. Hamidu and Aja M. Rieger and Gaylene M Fasenko and Daniel R. Barreda},
  journal={Poultry science},
  year={2010},
  volume={89 5},
  pages={901-9}
}
We describe a method of isolating blastodermal cells for characterization through flow cytometry. Particular attention was placed on cell viability and integrity issues faced by conventional protocols. The method allowed us to examine mechanisms behind cellular death. Our protocol was optimized by the spatial resolution of the ImageStream multispectral imaging flow cytometer. Overall, the technique provides both quantitative and qualitative information on blastodermal cells. The methodology was… CONTINUE READING