Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions.

Abstract

Transcriptase activity was dissociated from vesicular stomatitis virions by highionic-strength buffer containing Triton X-100. Considerable enzyme activity could be restored by recombining inactive sedimentable and nonsedimentable virion fractions. Reconstituted transcriptase activity was dependent on the presence of all four nucleoside triphosphates and… (More)

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