Dissimilatory nitrite reductase genes from autotrophic ammonia-oxidizing bacteria.

@article{Casciotti2001DissimilatoryNR,
  title={Dissimilatory nitrite reductase genes from autotrophic ammonia-oxidizing bacteria.},
  author={Karen L Casciotti and Bess Ward},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 5},
  pages={2213-21}
}
The presence of a copper-containing dissimilatory nitrite reductase gene (nirK) was discovered in several isolates of beta-subdivision ammonia-oxidizing bacteria using PCR and DNA sequencing. PCR primers Cunir3 and Cunir4 were designed based on published nirK sequences from denitrifying bacteria and used to amplify a 540-bp fragment of the nirK gene from Nitrosomonas marina and five additional isolates of ammonia-oxidizing bacteria. Amplification products of the expected size were cloned and… CONTINUE READING

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