Dissection of the core and auxiliary sequences in the vegetative replication origin of promiscuous plasmid RK2.

@article{Shah1995DissectionOT,
  title={Dissection of the core and auxiliary sequences in the vegetative replication origin of promiscuous plasmid RK2.},
  author={Dr. Seema S. Shah and M. A. Cross and D Porter and Christopher M. Thomas},
  journal={Journal of molecular biology},
  year={1995},
  volume={254 4},
  pages={608-22}
}
The vegetative replication origin (oriV) of promiscuous IncP plasmid RK2 can function in many Gram-negative bacterial species when supplied with the plasmid-encoded replication protein TrfA and host-encoded replication proteins including DnaA. Nine TrfA binding sites (iterons) are known, and also two DnaA binding sites, box 1, between TrfA iterons 4 and 5, and box 2, downstream of repeat 9. The deletion analysis presented here shows that the core oriV requires DnaA box 1 for function in… CONTINUE READING