Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.

@article{Mehta2016DissectionOM,
  title={Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.},
  author={Shalin B Mehta and Molly McQuilken and Patrick J. La Rivi{\`e}re and Patricia Occhipinti and Amitabh Verma and Rudolf Oldenbourg and Amy S Gladfelter and Tomomi Tani},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2016},
  volume={113 42},
  pages={E6352-E6361}
}
Regulation of order, such as orientation and conformation, drives the function of most molecular assemblies in living cells but remains difficult to measure accurately through space and time. We built an instantaneous fluorescence polarization microscope, which simultaneously images position and orientation of fluorophores in living cells with single-molecule sensitivity and a time resolution of 100 ms. We developed image acquisition and analysis methods to track single particles that interact… CONTINUE READING
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