Dissection of Axial-Pore Loop Function during Unfolding and Translocation by a AAA+ Proteolytic Machine.

@article{Iosefson2015DissectionOA,
  title={Dissection of Axial-Pore Loop Function during Unfolding and Translocation by a AAA+ Proteolytic Machine.},
  author={Ohad Iosefson and Adrian O. Olivares and Tania A. Baker and Robert T. Sauer},
  journal={Cell reports},
  year={2015},
  volume={12 6},
  pages={1032-41}
}
In the axial channels of ClpX and related hexameric AAA+ protein-remodeling rings, the pore-1 loops are thought to play important roles in engaging, mechanically unfolding, and translocating protein substrates. How these loops perform these functions and whether they also prevent substrate dissociation to ensure processive degradation by AAA+ proteases are open questions. Using ClpX pore-1-loop variants, single-molecule force spectroscopy, and ensemble assays, we find that the six pore-1 loops… CONTINUE READING

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