Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system.

@article{Guo2009DissectingTR,
  title={Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system.},
  author={Manman Guo and Hui Feng and Jun Yu Zhang and Wenqin Wang and Yi Wang and Yuqing Li and Chunhui Gao and Huanchun Chen and Weifeng Ding and Zheng-Guo He},
  journal={Genome research},
  year={2009},
  volume={19 7},
  pages={1301-8}
}
Sequence-specific DNA-binding transcription factors have widespread biological significance in the regulation of gene expression. However, in lower prokaryotes and eukaryotic metazoans, it is usually difficult to find transcription regulatory factors that recognize specific target promoters. To address this, we have developed in this study a new bacterial one-hybrid reporter vector system that provides a convenient and rapid strategy to determine the specific interaction between target DNA… CONTINUE READING