Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography

@article{Patla2010DissectingTM,
  title={Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography},
  author={Israel Patla and Tova Volberg and Nadav Elad and Vera C. Hirschfeld-Warneken and Carsten Grashoff and Reinhard F{\"a}ssler and Joachim Pius Spatz and Benjamin Geiger and Ohad Medalia},
  journal={Nature Cell Biology},
  year={2010},
  volume={12},
  pages={909-915}
}
Focal adhesions are integrin-based multiprotein complexes, several micrometres in diameter, that mechanically link the extracellular matrix with the termini of actin bundles. The molecular diversity of focal adhesions and their role in cell migration and matrix sensing has been extensively studied, but their ultrastructural architecture is still unknown. We present the first three-dimensional structural reconstruction of focal adhesions using cryo-electron tomography. Our analyses reveal that… 
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How the application of super-resolution techniques including single-molecule localization microscopy has revealed important new insights into focal adhesion structure and dynamics is discussed, including the first description of the three-dimensional nano-architecture of focal adhesions and of the dynamic exchange of integrins in focalAdhesions.
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Focal adhesion size uniquely predicts cell migration
  • Dong-Hwee Kim, D. Wirtz
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2013
TLDR
It is suggested that the mean size of focal adhesions robustly and precisely predicts cell speed independently of focalAdhesion surface density and molecular composition.
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The clusters appear to be very important elements in many cellular processes and can be considered as a critical functional module in both cell‐matrix and cell‐cell adhesions.
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