Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli.

@article{Fribourg2001DissectingTI,
  title={Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli.},
  author={S{\'e}bastien Fribourg and Christophe Romier and S. Werten and Y G Gangloff and Arnaud Poterszman and Dino Moras},
  journal={Journal of molecular biology},
  year={2001},
  volume={306 2},
  pages={363-73}
}
Using the human basal transcription factors TFIID and TFIIH as examples, we show that pairwise coexpression of polypeptides in Escherichia coli can be used as a tool for the identification of specifically interacting subunits within multiprotein complexes. We find that coexpression of appropriate combinations generally leads to an increase in the solubility and stability of the polypeptides involved, which means that large amounts of the resulting complexes can immediately be obtained for… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 27 extracted citations

Similar Papers

Loading similar papers…