Dissecting the functions of conserved prolines within transmembrane helices of the D2 dopamine receptor.

@article{Arnam2011DissectingTF,
  title={Dissecting the functions of conserved prolines within transmembrane helices of the D2 dopamine receptor.},
  author={Ethan B. Van Arnam and Henry A. Lester and Dennis A Dougherty},
  journal={ACS chemical biology},
  year={2011},
  volume={6 10},
  pages={1063-8}
}
G protein-coupled receptors (GPCRs) contain a number of conserved proline residues in their transmembrane helices, and it is generally assumed these play important functional and/or structural roles. Here we use unnatural amino acid mutagenesis, employing α-hydroxy acids and proline analogues, to examine the functional roles of five proline residues in the transmembrane helices of the D2 dopamine receptor. The well-known tendency of proline to disrupt helical structure is important at all sites… CONTINUE READING