Dissecting the Molecular Mechanisms of Intraflagellar Transport in Chlamydomonas

@article{Pedersen2006DissectingTM,
  title={Dissecting the Molecular Mechanisms of Intraflagellar Transport in Chlamydomonas},
  author={L. B. Pedersen and S. Geimer and J. Rosenbaum},
  journal={Current Biology},
  year={2006},
  volume={16},
  pages={450-459}
}
BACKGROUND The assembly and maintenance of eukaryotic cilia and flagella are mediated by intraflagellar transport (IFT), a bidirectional microtubule (MT)-based transport system. The IFT system consists of anterograde (kinesin-2) and retrograde (cDynein1b) motor complexes and IFT particles comprising two complexes, A and B. In the current model for IFT, kinesin-2 carries cDynein1b, IFT particles, and axonemal precursors from the flagellar base to the tip, and cDynein1b transports kinesin-2, IFT… Expand
Chlamydomonas: Intraflagellar Transport
Cilia/flagella are microtubule-based organelles emanating from the surface of most eukaryotic cells and accomplish motile and sensory functions. Malfunction of cilia results in a variety ofExpand
Identification and Characterization of Components of the Intraflagellar transport (IFT) Machinery: a Dissertation
Intraflagellar transport (IFT), the bi-directional movement of particles along the length of flagella, is required for flagellar assembly. The IFT particles are moved by kinesin II from the base toExpand
Single-Molecule Studies of Intraflagellar Transport in Chlamydomonas
TLDR
The results demonstrate the mechanism of Chlamydomonas gliding motility and suggest that IFT plays a major role in adhesion-induced ciliary signaling pathways, and propose new models for mixing and remodeling of IFT particles at the flagellar tip, coordination of I FT motors and flageLLar length control. Expand
The GTPase IFT27 is involved in both anterograde and retrograde intraflagellar transport
TLDR
It is demonstrated that a GTPase-competent IFT 27 is required for association to the IFT complex and that IFT27 plays a role in the cargo loading of the retrograde transport machinery. Expand
Intraflagellar transport: from molecular characterisation to mechanism.
TLDR
A comprehensive review of intraflagellar transport is presented, with particular emphasis on its molecular composition and mechanism of action. Expand
In vivo imaging of IFT in Chlamydomonas flagella.
TLDR
Kymograms, graphical representations of spatial position over time, provide a convenient way to analyze in vivo recordings of IFT and its cargoes and will be used to understand how flagella are assembled, maintained, and repaired. Expand
Probing the role of IFT particle complex A and B in flagellar entry and exit of IFT-dynein in Chlamydomonas
TLDR
Investigation of the role of complex A and complex B in the flagellar entry and exit of IFT-dynein indicates that complexes A and B play complementary but distinct roles for I FT-dynesin, with complex B carried into flagella and transported to the flagllar tip by kinesin II, where it is activated to drive the retrograde IFT back to the floater base. Expand
Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery
Intraflagellar transport (IFT) is a form of motor‐dependent cargo transport that is essential for the assembly, maintenance, and length control of cilia, which play critical roles in motility,Expand
Dynein and intraflagellar transport.
TLDR
The basic function of cytoplasmic dynein 2 as the motor for retrograde IFT is now well established, and its general molecular architecture can be accurately predicted, however, much remains to be learned about this unique and important dyneIn. Expand
Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor
Intraflagellar transport (IFT) is the bi-directional movement of particles along the length of axonemal outer doublet microtubules and is needed for the assembly and maintenance of eukaryotic ciliaExpand
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