Dissecting self-renewal in stem cells with RNA interference

@article{Ivanova2006DissectingSI,
  title={Dissecting self-renewal in stem cells with RNA interference},
  author={Natalia Ivanova and Radu Dobrin and Rong Lu and Iulia Kotenko and John Levorse and Christina J Decoste and Xenia L. Schafer and Yi Lun and Ihor Lemischka},
  journal={Nature},
  year={2006},
  volume={442},
  pages={533-538}
}
We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem cells. We use short hairpin RNA (shRNA) loss-of-function techniques to downregulate a set of gene products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven genes for which shRNA-mediated depletion negatively affects self-renewal, including four genes with previously unrecognized roles in self-renewal… CONTINUE READING

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