Dissecting immunity by germline mutagenesis

  title={Dissecting immunity by germline mutagenesis},
  author={Onjee Choi and S. Rutschmann},
The last decades have seen numerous approaches being used to decipher biological phenomena, notably the strategies we employ to defend ourselves against pathogenic attacks. From microarrays to genetics to computing technologies, all have supported a better but not yet comprehensive understanding of the pathways regulating our immune system. Limitations are notably exemplified by cases of immune deficiencies in humans that often result in high susceptibility to infections or even death, without… Expand
4 Citations
Understanding Immunity through the Lens of Disease Ecology.
  • S. Hedrick
  • Biology, Medicine
  • Trends in immunology
  • 2017
It is proposed that the progression and outcome of disease-causing host-parasite interactions will be more clearly understood through a focus on disease ecology. Expand
Trade-Off Theory and the Evolution of Parasite Virulence
As we describe the immune system in ever more exquisite detail, we might find that no matter how successful, this approach will not be sufficient to understand the spread of infectious agents, theirExpand
N‐Ethyl‐N‐nitrosourea mutagenesis in the mouse provides strong genetic and in vivo evidence for the role of the Caspase Recruitment Domain (CARD) of CARD‐MAGUK1 in T regulatory cell development
Strong genetic evidence of a scaffold function for the Caspase Recruitment Domain (CARD) of the TCR signalling protein CARD‐MAGUK1 (CARMA1) in nTreg cell development in vivo is provided. Expand


Dissecting innate immunity by germline mutagenesis
For both animal models, forward genetics has been instrumental in the deciphering of innate immunity and has greatly improved the understanding of how the authors respond to invading pathogens. Expand
Genetic analysis of host resistance: Toll-like receptor signaling and immunity at large.
The positional cloning of a spontaneous mutation that caused lipopolysaccharide resistance and susceptibility to Gram-negative infection led directly to the understanding that Toll-like receptors (TLRs) are essential sensors of microbial infection. Expand
Analysis of the MCMV resistome by ENU mutagenesis
Identification of the Domino mutation demonstrates that an in vivo MCMV susceptibility screen is feasible and illustrates how it can provide insight into the resistome, consistent with the interpretation that certain protein(s) unrelated to IFN production or signaling are more important than IFNs with regard to their net antiviral effects. Expand
Mouse ENU mutagenesis.
O Ongoing mouse ENU mutagenesis experiments are generating a treasure trove of new mutations to allow an in-depth study of a single gene, a chromosomal region or a biological system. Expand
From phenomenon to phenotype and from phenotype to gene: forward genetics and the problem of sepsis.
The greatest challenge confronting all fields in biology is to establish correspondence between genes and discrete biologic functions, which requires the solution of phenotypic puzzles, which, in turn, requires the creation of phenotypes. Expand
Human genetics of infectious diseases: between proof of principle and paradigm.
Although many studies have provided proof of principle that infectious diseases may result from various types of inborn errors of immunity, the genetic determinism of most infectious diseases in most patients remains unclear. Expand
Identification of two new Pmp22 mouse mutants using large-scale mutagenesis and a novel rapid mapping strategy.
This is the first report of the generation of a clinically relevant neurological mutant and its rapid genetic characterization from a large-scale mutagenesis screen for dominant phenotypes in the mouse, and validates the use of large- scale screens to generate desired clinical phenotype in mice. Expand
A Deficiency in Nucleoside Salvage Impairs Murine Lymphocyte Development, Homeostasis, and Survival
A deficiency in deoxycytidine kinase is described, one of the major enzymes of the nucleoside salvage pathway, which affects peripheral T cell homeostatic proliferation and survival and establishes dCK as a new regulator of hematopoietic integrity and lymphocyte quiescence and survival. Expand
Jinx, an MCMV susceptibility phenotype caused by disruption of Unc13d: a mouse model of type 3 familial hemophagocytic lymphohistiocytosis
Jinx is described, an N-ethyl-N-nitrosourea-induced MCMV susceptibility mutation that permits unchecked proliferation of the virus, causing death in mice, which suggests the existence of a specific infectious trigger of FHL3 in humans. Expand
The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9
'triple D' (3d), a recessive N-ethyl-N-nitrosourea-induced mutation and phenotype in which no signaling occurs via the intracellular Toll-like receptors 3, 7 and 9, suggests communication between these organellar systems. Expand