Dissecting cell type-specific metabolism in pancreatic ductal adenocarcinoma

  title={Dissecting cell type-specific metabolism in pancreatic ductal adenocarcinoma},
  author={Allison N. Lau and Zhaoqi Li and Laura V Danai and Anna M. Westermark and Alicia M. Darnell and Raphael Ferreira and Vasilena Gocheva and Sharanya Sivanand and Evan C. Lien and Kiera M Sapp and Jared R Mayers and Giulia Biffi and Christopher R Chin and Shawn Michael Davidson and David A. Tuveson and Tyler Jacks and Nicholas J. Matheson and Omer H Yilmaz and Matthew G. Vander Heiden},
Tumors are composed of many different cell types including cancer cells, fibroblasts, and immune cells. Dissecting functional metabolic differences between various cell types within a mixed population can be limited by the rapid turnover of metabolites relative to the time needed to isolate cells. To overcome this challenge, we traced isotope-labeled nutrients into macromolecules that turn over more slowly than metabolites. This approach was used to assess differences between cancer cell and… Expand
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