Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor

  title={Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor},
  author={Aaron F. Straight and Amy Cheung and John Limouze and Irene Chen and Nicholas James Westwood and James R. Sellers and Timothy J. Mitchison},
  pages={1743 - 1747}
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated… 

Equatorial Retention of the Contractile Actin Ring by Microtubules During Cytokinesis

It is proposed that late-mitotic microtubular structures located at the division plane contribute to a checkpoint control that retains the equatorial position of the ring when progression through cytokinesis is delayed.

Rho GTPases as regulators of mitosis and cytokinesis in mammalian cells

The molecular mechanisms regulating the spatial and temporal activation of RhoA and Cdc42 during mitosis are reviewed, and how this is critical for mitotic progression and completion are reviewed.

The Contribution of Polar Cortical Actin and Microtubule to Cytokinesis at Early Anaphase

The findings showed that disturbed polar cortical cytoskeleton not only inhibited initiation of cytokinesis but also influenced the position of furrow, which suggested the definitive roles of global coordination of cortical activities at early anaphase.

The actin-binding and bundling protein, EPLIN, is required for cytokinesis

The actin-binding protein, EPLIN, locates to the cleavage furrow during cytokinesis and this is possibly via association with the contractile ring components, myosin II, and the septin, Sept2 and their regulatory small GTPases, RhoA and Cdc42, respectively.

A microtubule-dependent zone of active RhoA during cleavage plane specification

It is shown that active RhoA concentrates in a precisely bounded zone before cytokinesis and is independent of actin assembly, and it is concluded that microtubules specify the cytokinetic apparatus via a dynamic zone of local RHoA activity.

Cell polarization during monopolar cytokinesis

It is proposed that feedback loops between cortical furrow components and microtubules promote symmetry breaking during monopolar cytokinesis and regional specialization of the cytoskeleton during normal bipolar cytokineis.

Regulation of Actin Network Dynamics by Actomyosin Contractility

This chapter explores the role of actomyosin contractility in the regulatory process of actin dynamics and presents a mechanical regulatory pathway that highlights the roles of mechanical factors in the regulation of actIn dynamics in migrating cells.

Microtubule plus-end tracking proteins and their roles in cell division.




Animal cell cytokinesis.

  • M. Glotzer
  • Biology
    Annual review of cell and developmental biology
  • 2001
An overview of the literature on cytokinesis is provided with a particular emphasis on the molecular pathways involved in the division of animal cells.

Both midzone and astral microtubules are involved in the delivery of cytokinesis signals

It is suggested that cultured cells use midzone micro Tubules as the primary signaling pathway for cytokinesis, whereas embryos, with their stockpile of cytoplasmic proteins and large sizes, rely primarily on astral microtubules.

Relationships between the central spindle and the contractile ring during cytokinesis in animal cells

It is suggested that the interaction between central spindle microtubules and cortical actin filaments leads to two early events crucial for cytokinesis: the positioning of the contractile ring, and the stabilization of the plus ends of the interdigitating micro Tubule scaffold that comprise thecentral spindle.

Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex

The cDNA sequence and localization of a protein first identified by actin filament chromatography of Drosophila embryo extracts as ABP8 is reported and it is speculated that anillin plays a role in organizing and or stabilizing the cleavage furrow and other cell cycle regulated, contractile domains of the actin cytoskeleton.

Simultaneous inhibition of contractile ring and central spindle formation in mammalian cells treated with cytochalasin B

Data suggest that the presence of a correctly assembled contractile ring is essential for the formation and persistence of the central spindle during ana-telophase and provide further support for the idea of a strong co-operative interaction between these two structures during cytokinesis.