Dissecting Oct3/4-Regulated Gene Networks in Embryonic Stem Cells by Expression Profiling

@article{Matoba2006DissectingOG,
  title={Dissecting Oct3/4-Regulated Gene Networks in Embryonic Stem Cells by Expression Profiling},
  author={R. Matoba and H. Niwa and S. Masui and S. Ohtsuka and M. G. Carter and A. Sharov and M. Ko},
  journal={PLoS ONE},
  year={2006},
  volume={1}
}
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation (ChIP) assays, this analysis identifies not only primary downstream targets of Oct3/4, but also secondary or tertiary targets. Furthermore, the analysis also reveals that downstream target genes are… Expand
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Roles of OCT4 in pathways of embryonic development and cancer progression
  • S. Patra
  • Medicine, Biology
  • Mechanisms of Ageing and Development
  • 2020
TLDR
A consensus is emerging that chromatin organization and epigenetic landscape play crucial roles for the interactions of transcription factors, including OCT4 with the promoters and/or regulatory sequences of genes associated with human embryonic development and that when the epigenome niche is deregulated OCT4 helps in cancer progression. Expand
Auxin-degron system identifies immediate mechanisms of Oct4
TLDR
A rapid protein-level Oct4 depletion system is developed that demonstrates that the immediate downstream response to loss of Oct4 is reduced expression of key pluripotency factors, and suggests that expression of trophectoderm markers is a subsequent event. Expand
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