Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single-cell assays.

Abstract

Postprandial blood glucose homeostasis is regulated by an insulin-stimulated increase in glucose transport into muscle and fat tissues via glucose transporter isoform 4 (GLUT4). In the basal state, this constitutively recycling membrane protein predominantly resides intracellularly. In order to achieve the insulin-stimulated increase in glucose flux, GLUT4 increases its cell surface abundance at the expense of preformed intracellular depots. By confocal microscopy of cultured L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc), we can visualize the two arms of GLUT4 traffic: exocytosis (movement to the cell surface) and endocytosis (internalization from the cell surface).

Cite this paper

@article{Antonescu2008DissectingGT, title={Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single-cell assays.}, author={Costin N Antonescu and Varinder K Randhawa and Amira Klip}, journal={Methods in molecular biology}, year={2008}, volume={457}, pages={367-78} }