Disruption of the internal thioester bond in the third component of complement (C3) results in the exposure of neodeterminants also present on activation products of C3. An analysis with monoclonal antibodies.

@article{Hack1988DisruptionOT,
  title={Disruption of the internal thioester bond in the third component of complement (C3) results in the exposure of neodeterminants also present on activation products of C3. An analysis with monoclonal antibodies.},
  author={C. Erik Hack and J Paardekooper and R. J. T. Smeenk and J. J. Abbink and Anke J. M. Eerenberg and Jan H. Nuijens},
  journal={Journal of immunology},
  year={1988},
  volume={141 5},
  pages={1602-9}
}
Hydrolysis of the internal thioester bond in native C3 is thought to be a key event in initiating the alternative pathway of C activation, because the resulting C3(H2O) acquires "C3b-like" properties. Therefore, disruption of the internal thioester bond is probably accompanied by conformational changes in the C3 molecule. In this study, we demonstrate that such conformational changes indeed occur; 7 of the 19 mAb raised against C3 or C3 activation products recognized epitopes exposed on C3(H2O… CONTINUE READING