Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression.

@article{Guillemot2004DisruptionOT,
  title={Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression.},
  author={Laurent Guillemot and Eva Hammar and Christian Kaister and Jorge Ritz and Doroth{\'e}e Caille and Lionel Jond and Christoph Bauer and Paolo Meda and Sandra Citi},
  journal={Journal of cell science},
  year={2004},
  volume={117 Pt 22},
  pages={5245-56}
}
Cingulin, a component of vertebrate tight junctions, contains a head domain that controls its junctional recruitment and protein interactions. To determine whether lack of junctional cingulin affects tight-junction organization and function, we examined the phenotype of embryoid bodies derived from embryonic stem cells carrying one or two alleles of cingulin with a targeted deletion of the exon coding for most of the predicted head domain. In homozygous (-/-) embryoid bodies, no full-length… CONTINUE READING