Disruption of the Rad9/Rad1/Hus1 (9–1–1) complex leads to checkpoint signaling and replication defects

@article{Bao2004DisruptionOT,
  title={Disruption of the Rad9/Rad1/Hus1 (9–1–1) complex leads to checkpoint signaling and replication defects},
  author={Shilai Bao and Tao Lu and Xin Wang and Huyong Zheng and Lei E. Wang and Qingyi Wei and Walter N. Hittelman and Lei Li},
  journal={Oncogene},
  year={2004},
  volume={23},
  pages={5586-5593}
}
The checkpoint sliding-clamp complex, Rad9/Rad1/Hus1, plays a critical role during initiation of checkpoint signals in response to DNA damage and replication disruption. We investigated the impact of loss of Rad1 on checkpoint function and on DNA replication in mammalian cells. We show that RAD1 is an essential gene for sustained cell proliferation and that loss of Rad1 causes destabilization of Rad9 and Hus1 and consequently disintegration of the sliding-clamp complex. In Rad1-depleted cells… CONTINUE READING