Disruption of the PU.1 gene in chicken B lymphoma DT40 cells and its effect on reported target gene expression.


Using avian B lymphoma-derived DT40 cells, we disrupted a gene encoding the transcription factor PU.1. The mutant mRNA codes for a protein incapable of functioning as a transcription factor because of the deletion of the protein's DNA-binding domain. The absence of a functional PU.1 protein in the mutant cells was confirmed by Western blotting and… (More)