Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans

  title={Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans},
  author={H. Habe and T. Fukuoka and T. Morita and D. Kitamoto and T. Yakushi and K. Matsushita and K. Sakaki},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={1391 - 1395}
Dihydroxyacetone (DHA) production from glycerol by Gluconobacter oxydans is an industrial form of fermentation, but some problems exist related to microbial DHA production. For example, glycerol inhibits DHA production and affects its biological activity. G. oxydans produces both DHA and glyceric acid (GA) from glycerol simultaneously, and membrane-bound glycerol dehydrogenase and membrane-bound alcohol dehydrogenases are involved in the two reactions, respectively. We discovered that the G… Expand
Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003.
The transcription levels of two terminal ubiquinol oxidases in the respiratory chain of the engineered strain G. oxydans-adhABS were up-regulated by 13.4- and 3.8-fold, which effectively enhanced the oxygen uptake rate, resulting in higher resistance to acid. Expand
Effect of membrane-bound aldehyde dehydrogenase-encoding gene disruption on glyceric acid production in Gluconobacter oxydans.
An aldH-disrupted mutant of G. oxydans was constructed, which was unable to produce acetic acid from ethanol, but was able to produce GA at a level approximately half that of the wildtype strain, suggesting the involvement of another ALDH in GA production. Expand
Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way.
All strains of GDHE, GAT and GAN cultivated on glucose showed enhanced catalytic capacity than those grown on sorbitol, indicating a favorable prospect of using glucose as carbon source to reduce the cost in industrial production. Expand
Research of 1,3-Dihydroxyacetone Production by Overexpressing Glycerol Transporter and Glycerol Dehydrogenase
Abstract1,3-Dihydroxyacetone (DHA), a natural ketose, is widely used in the chemical, cosmetic, and pharmaceutical industries. The current method for DHA production is Gluconobacter oxydans (G.Expand
Effective improvement of the activity of membrane-bound alcohol dehydrogenase by overexpression of adhS in Gluconobacter oxydans
Investigating the roles of adhS, which encodes the AdhS subunit of membrane-bound alcohol dehydrogenase (mADH) in Gluconobacter oxydans DSM2003, and overexpression effectively improved mADH activity, indicating that for m ADH, adhH might be a limiting component. Expand
Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase in the bio-oxidation of alcohols in Gluconobacter oxydans DSM 2003
In this study a new insight was provided to understand the functions of membrane-bound alcohol dehydrogenase (mADH) and aldehyde dehydrogenase (mALDH) in the bio-oxidation of primary alcohols, diolsExpand
Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol
Results strongly suggest that the observed decrease in GA production by Gluconobacter spp. Expand
Three ATP-dependent phosphorylating enzymes in the first committed step of dihydroxyacetone metabolism in Gluconobacter thailandicus NBRC3255.
All three DHA kinases, DhaK, DerK, and GlpK, are involved in DHA metabolism of G. thailandicus, and it is concluded that Phosphorylation rather than reduction is the first committed step in D HA metabolism. Expand
Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621
DHA was shown to inhibit the mitotic activity of G. oxydans bacteria even at low concentrations, whereas at the concentration of 70 g L−1, it made cell divisions impossible, and the applied pH values of the culture media did not inhibit the growth of the analyzed strain. Expand
Characterization of membrane-bound dehydrogenases from Gluconobacter oxydans 621H via whole-cell activity assays using multideletion strains
There are less membrane-bound dehydrogenases in G. oxydans 621H than expected and that the deletion of all of them is not lethal for the organism, demonstrating that general rules can be established for some of the enzymes and extended the known substrate spectra of some enzymes. Expand


Biotransformation of glycerol to dihydroxyacetone by recombinant Gluconobacter oxydans DSM 2343
The effect on sldAB overexpression on the G. oxydans transcriptome was investigated and two genes involved in glycerol metabolism, as well as a regulator of the LysR family were identified. Expand
Production of Gluconobacter oxydans Cells from Low‐cost Culture Medium for Conversion of Glycerol to Dihydroxyacetone
The corn meal hydrolysate and corn steep liquor medium was similar in performance to a nutrient‐rich medium, but the cost of production was only 15% of that cultured in sorbitol and yeast extract medium. Expand
Kinetic study and optimisation of the production of dihydroxyacetone from glycerol using Gluconobacter oxydans
Abstract Kinetic parameters were determined during dihydroxyacetone (DHA) production by Gluconobacter oxydans in batch culture with different glycerol concentrations (50 and 100 g litre−1).Expand
Production of Glyceric Acid by Gluconobacter sp. NBRC3259 Using Raw Glycerol
Gluconobacter sp. NBRC3259 converted glycerol to glyceric acid (GA). The enantiomeric composition of the GA produced was a mixture of DL-forms with a 77% enantiomeric excess of D-GA. After cultureExpand
Microbial Production of Glyceric Acid, an Organic Acid That Can Be Mass Produced from Glycerol
A method for the efficient biotechnological production of GA as a target compound for new surplus glycerol applications in the biodiesel and oleochemical industries is developed and suggests that mADH is involved in GA production by acetic acid bacteria. Expand
Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans
An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented and it was observed that the maximum achievable productivity had not been reached yet. Expand
Glycerol inhibition of growth and dihydroxyacetone production byGluconobacter oxydans
The evidence, kinetic aspects, and modelization of the inhibitory effect of glycerol on dihydroxyacetone (DHA) production byGluconobacter oxydans have been studied. The comparison of the maximalExpand
Inhibitory effect of dihydroxyacetone onGluconobacter oxydans: Kinetic aspects and expression by mathematical equations
SummaryMicrobial conversion of glycerol into dihydroxyacetone (DHA) byGluconobacter oxydans was subjected to inhibition by excess substrate. Comparison of cultures containing increasing initial DHAExpand
Biotechnological production of d-glyceric acid and its application
This mini-review provides recent information about the biotechnological production of a glycerol derivative, d-glyceric acid (d-GA), and its possible applications. Expand
Product yield and by-product formation in glycerol conversion to dihydroxyacetone by Gluconobacter oxydans
Abstract Dihydroxyacetone was produced by the strain Gluconobacter oxydans CCM 1783 (= ATCC 621), which is the most frequently cited strain for this conversion. The influence of the oxygenExpand