Disruption of murine alpha-enolase by a retroviral gene trap results in early embryonic lethality.

@article{Couldrey1998DisruptionOM,
  title={Disruption of murine alpha-enolase by a retroviral gene trap results in early embryonic lethality.},
  author={Chris Couldrey and Mark B. L. Carlton and Jack Ferrier and William H Colledge and Martin J. Evans},
  journal={Developmental dynamics : an official publication of the American Association of Anatomists},
  year={1998},
  volume={212 2},
  pages={284-92}
}
Gene trapping with the retroviral ROSA beta geo vector was used to generate lines of mice carrying disrupted genes. Both cDNA and genomic flanks have been cloned from a number of these lines. One mutation has been shown to disrupt the alpha-enolase gene by insertion of the splice-trap vector into the first intron. In adult mice, lacZ expression was detected only in testes. Embryonic expression was detected from 10.5-day postcoitum embryos and was seen as a diffuse staining pattern over much of… CONTINUE READING