Disruption of Homocitrate Synthase Genes in Candida albicans Affects Growth But Not Virulence

@article{Kur2010DisruptionOH,
  title={Disruption of Homocitrate Synthase Genes in Candida albicans Affects Growth But Not Virulence},
  author={Krzysztof Kur and Iwona Gabriel and Joachim Morschh{\"a}user and Francesco Barchiesi and Elisabetta Spreghini and Sławomir Milewski},
  journal={Mycopathologia},
  year={2010},
  volume={170},
  pages={397-402}
}
Two genes, LYS21 and LYS22, encoding isoforms of homocitrate synthase, an enzyme catalysing the first committed step in the lysine biosynthetic pathway, were disrupted in Candida albicans using the SAT1 flipper strategy. The double null lys21Δ/lys22Δ mutant lacked homocitrate synthase activity and exhibited lysine auxotrophy in minimal media that could be fully rescued by the addition of 0.5–0.6 mM l-lysine. On the other hand, its virulence in vivo in the model of disseminated murine… CONTINUE READING