Disruption of GW bodies impairs mammalian RNA interference

@article{Jakymiw2005DisruptionOG,
  title={Disruption of GW bodies impairs mammalian RNA interference},
  author={Andrew Jakymiw and Shangli Lian and Theophany Eystathioy and Songqing Li and Minoru Satoh and John C. Hamel and Marvin J. Fritzler and Edward K L Chan},
  journal={Nature Cell Biology},
  year={2005},
  volume={7},
  pages={1267-1274}
}
The GW182 RNA-binding protein was initially shown to associate with a specific subset of mRNAs and to reside within discrete cytoplasmic foci named GW bodies (GWBs). GWBs are enriched in proteins that are involved in mRNA degradation. Recent reports have shown that exogenously introduced human Argonaute-2 (Ago2) is also enriched in GWBs, indicating that RNA interference function may be somehow linked to these structures. In this report, we demonstrate that endogenous Ago2 and transfected small… Expand
The role of GW/P-bodies in RNA processing and silencing
TLDR
Evidence indicates that both passenger and guide strands of short-interfering RNA duplexes can localize to GW bodies, thereby indicating that RNA-induced silencing complexes may be activated within these cytoplasmic centers. Expand
A C-terminal silencing domain in GW182 is essential for miRNA function.
TLDR
It is indicated that miRNA silencing in animal cells is mediated by AGO1 in complex with GW182, and that P-body localization is not required for silencing. Expand
Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2.
TLDR
It is shown that upon transfection into cells, siRNAs rapidly localize to P-bodies in their native double-stranded conformation, as indicated by fluorescence resonance energy transfer imaging and that Ago2 is at least in part responsible for this siRNA localization pattern, indicating RISC involvement. Expand
Formation of GW bodies is a consequence of microRNA genesis
TLDR
It is reported that endogenous let‐7 miRNA co‐precipitates with the GW182 protein complex and knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Expand
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay
TLDR
The findings show that miRNA function is effected by AGO1–GW182 complexes and the role of GW182 in silencing goes beyond promoting deadenylation. Expand
Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development
TLDR
The characterized Drosophila melanogaster homologue of the human GW182 protein family is named Gawky (GW), suggesting that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development. Expand
Function of GW182 and GW bodies in siRNA and miRNA pathways.
GW182 is an 182 kDa protein with multiple glycine/tryptophan repeats (GW or WG) playing a central role in siRNA- and miRNA-mediated gene silencing. GW182 interacts with its functional partnerExpand
Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies.
TLDR
It is proposed that RNAi is a key regulatory mechanism for the assembly of GWB, and in some cases, GWB may serve as markers for RNAi in mammalian cells. Expand
GW body disassembly triggered by siRNAs independently of their silencing activity
TLDR
It is proposed that some siRNAs targeting genes encoding proteins unrelated to mRNA metabolism affect mRNA metabolism so as to diminish the amount of mRNA directed to the GW bodies. Expand
Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody.
TLDR
It is demonstrated that the anti-Ago2 monoclonal antibody 4F9 recognized GWBs in a cell cycle dependent manner and was capable of capturing miRNAs associated with Ago2, the effector protein of RNAi. Expand
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TLDR
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TLDR
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TLDR
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