Disrupting Flavone Synthase II Alters Lignin and Improves Biomass Digestibility1[OPEN]

@article{Lam2017DisruptingFS,
  title={Disrupting Flavone Synthase II Alters Lignin and Improves Biomass Digestibility1[OPEN]},
  author={Pui Ying Lam and Yuki Tobimatsu and Yuri Takeda and Shiro Suzuki and Masaomi Yamamura and Toshiaki Umezawa and Clive Lo},
  journal={Plant Physiology},
  year={2017},
  volume={174},
  pages={972 - 985}
}
Disruption of the flavone synthase II gene in rice results in an altered cell wall lignin incorporating naringenin as a novel flavonoid component and improves biomass saccharification efficiency. Lignin, a ubiquitous phenylpropanoid polymer in vascular plant cell walls, is derived primarily from oxidative couplings of monolignols (p-hydroxycinnamyl alcohols). It was discovered recently that a wide range of grasses, including cereals, utilize a member of the flavonoids, tricin (3′,5… Expand
OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls.
TLDR
Lignin-enriched transgenic rice is generated via targeted mutagenesis of the transcriptional repressor OsMYB108 using CRISPR/Cas9-mediated genome editing and it was revealed that the mutant cell walls were preferentially enriched in γ-p-coumaroylated and tricin lignin units, both of which are typical and unique components in grass lignins. Expand
OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls
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The data demonstrate that C3'H expression is an important determinant not only of lignin content and composition but also of the degree of cell wall cross-linking. Expand
Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice
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Manipulation of a gene encoding coniferaldehyde 5-hydroxylase leads to substantial alterations in lignin structure in rice cell walls, identifying a promising genetic engineering target for improving grass biomass utilization. Expand
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TLDR
It is suggested that CAld5H is mainly involved in theProduction of non-γ-p-coumaroylated S lignin units, common in both eudicots and grasses, but not in the production of grass-specific γ-p -cou maroylatedS units in rice. Expand
Distinctively altered lignin biosynthesis by site‐modification of OsCAD2 for enhanced biomass saccharification in rice
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  • Chemistry
  • 2020
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Grass secondary cell walls, Brachypodium distachyon as a model for discovery.
TLDR
The regulatory aspects of cell wall biosynthesis are largely overlapping with those of eudicots, but salient differences among species have been resolved that begin to identify the determinants that define grass cell walls. Expand
Double knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice.
TLDR
The results provide evidence that both OsWRKY36 andOsWRKY102 are associated with repression of rice lignification, and relative abundances of guaiacyl and p-coumarate units were slightly higher and lower, respectively, in the WRKY mutant lignins compared with those in the wild-type lign ins. Expand
Apigenin produced by maize flavone synthase I and II protects plants against UV-B-induced damage.
TLDR
It is shown that maize has two FNS-type enzymes that participate in the synthesis of apigenin, conferring protection against UV-B radiation. Expand
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