Disposition and intracellular activity of azithromycin in human THP-1 acute monocytes.

@article{Hall2002DispositionAI,
  title={Disposition and intracellular activity of azithromycin in human THP-1 acute monocytes.},
  author={Iris H. Hall and Ute E. Schwab and Elaine S. Ward and J David Butts and Eric T Wolford and Timothy J. Ives},
  journal={International journal of antimicrobial agents},
  year={2002},
  volume={20 5},
  pages={348-60}
}
Uptake of [14C]-azithromycin into THP-1 human monocytes was determined at pH 7.4, 6.8 or 5.5 over 4-log antibiotic concentrations for 24 h under a number of conditions. Stimulation of cells was with bacteria, latex beads, lipopolysaccharide (LPS), or zymogen A. Subcellular organelle disposition was determined after isolation by ultracentrifugation or sucrose gradients. Hydrolytic enzyme activities and mediators of intracellular inflammation (IL-1, IL-6, IL-8, and TNFalpha) were assessed… CONTINUE READING