Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines

@article{Georgiou1997DisplayOH,
  title={Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines},
  author={George D. Georgiou and Christos Stathopoulos and Patrick S. Daugherty and Amiya Nayak and Brent L. Iverson and Roy Curtiss},
  journal={Nature Biotechnology},
  year={1997},
  volume={15},
  pages={29-34}
}
In recent years there has been considerable progress towards the development of expression systems for the display of heterologous polypeptides and, to a lesser extent, oligosaccharides on the surface of bacteria or yeast. The availability of protein display vectors has in turn provided the impetus for a range of exciting technologies. Polypepttde libraries can be displayed in bacteria and screened by cell sorting techniques, thus simplifying the isolation of proteins with high affinity for… CONTINUE READING