Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B

@article{Wang2016DisplayOF,
  title={Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B},
  author={Pan Wang and Jie He and Yufei Sun and Matthew P. Reynolds and Li Zhang and Shuangyan Han and Shuli Liang and Haixin Sui and Ying Lin},
  journal={Applied Microbiology and Biotechnology},
  year={2016},
  volume={100},
  pages={5883-5895}
}
To modify the Pichia pastoris cell surface, two classes of hydrophobins, SC3 from Schizophyllum commune and HFBI from Trichoderma reesei, were separately displayed on the cell wall. There was an observable increase in the hydrophobicity of recombinant strains. Candida antarctica lipase B (CALB) was then co-displayed on the modified cells, generating strains GS115/SC3–61/CALB-51 and GS115/HFBI-61/CALB-51. Interestingly, the hydrolytic and synthetic activities of strain GS115/HFBI-61/CALB-51… CONTINUE READING
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