Dispersion and characterisation of mast cells from human skin.

  title={Dispersion and characterisation of mast cells from human skin.},
  author={Robert Christopher Benyon and M K Church and Lizzie Clegg and Stephen T. Holgate},
  journal={International archives of allergy and applied immunology},
  volume={79 3},
A technique is described for the enzymatic dispersion of mast cells in high yield from human infant foreskin. Dispersed mast cells exhibit high viability as assessed by light microscopy, low spontaneous histamine release, and survival in culture. Dispersed mast cells release histamine in response to immunological stimulation and synthetic secretagogues including ionophore A23187, compound 48/80 and poly-L-lysine. Reactivity to these stimuli indicates that cutaneous mast cells differ in their… CONTINUE READING
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