Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination.

@article{Silver1993DispensableSM,
  title={Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination.},
  author={Daniel P. Silver and Eugenia Spanopoulou and Richard c. Mulligan and David Baltimore},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 13},
  pages={6100-4}
}
As a probe of whether RAG-1 and RAG-2 gene products activate other genes or form part of the recombinase itself, certain mutants of the RAG genes were assayed for their ability to activate variable-diversity-joining region [V(D)J] recombination in a plasmid substrate in fibroblasts. The results indicate that the N-terminal one-third of RAG-1, including a zinc-finger-like domain, and an acidic domain of RAG-2 are dispensable for activating V(D)J recombination in a fibroblast, although they… CONTINUE READING