Disparate differentiation in mouse hemopoietic colonies derived from paired progenitors.

@article{Suda1984DisparateDI,
  title={Disparate differentiation in mouse hemopoietic colonies derived from paired progenitors.},
  author={Toshio Suda and Jun Suda and Minetaro Ogawa},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 8},
  pages={2520-4}
}
We analyzed the differentiation of murine hemopoietic colonies derived from paired progenitors in culture. Single progenitors were isolated by use of a micromanipulation technique from blast cell colonies cultured from the spleens of 5-fluorouracil-treated mice. Eighteen to 24 hr later, the paired progenitors were separated with a micromanipulator and cultured in methylcellulose medium containing erythropoietin and pokeweed-mitogen spleen cell conditioned medium. Six to nine days later, the two… CONTINUE READING
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