Disordered water within a hydrophobic protein cavity visualized by x-ray crystallography.

  title={Disordered water within a hydrophobic protein cavity visualized by x-ray crystallography.},
  author={Bo Yu and Michael Blaber and Angela M. Gronenborn and G Marius Clore and Donald L. D. Caspar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={96 1},
Water in the hydrophobic cavity of human interleukin 1beta, which was detected by NMR spectroscopy but was invisible by high resolution x-ray crystallography, has been mapped quantitatively by measurement and phasing of all of the low resolution x-ray diffraction data from a single crystal. Phases for the low resolution data were refined by iterative density modification of an initial flat solvent model outside the envelope of the atomic model. The refinement was restrained by the condition… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 29 extracted citations


Publications referenced by this paper.
Showing 1-4 of 4 references

XPLOR 3.1: A System for X-ray Crystallography and NMR (Yale

  • A. T. Brünger
  • 1992
Highly Influential
5 Excerpts

Yu et al

  • C. Kolb, Bogart, R. RAYSHADE 4.0 Princeton Univ., Princeton, NJ. 108 Biophysics
  • Proc. Natl. Acad. Sci. USA 96
  • 1999
1 Excerpt

Proteins Struct

  • B. W. Matthews, A. G. Morton, F. W. Dahlquist
  • Funct . Genet .
  • 1992

Molecular and Cellular Biology of IL-1, TNF and Lipocortins in Inflammation and Differentiation, ed

  • A. C. Treharne, D. H. Ohlendorf, P. C. Weber, J. J. Wendoloski, F. R. Salemme
  • 1990
1 Excerpt

Similar Papers

Loading similar papers…